Explore more about Optimized settings for Transcreener TR-FRET assay on SpectraMax iD5 and SpectraMax i3x readers. The spectra analysis and K d were determined using Sigmaplot 14. SpectraMax Paradigm Cisbio HTRF Detection Cartridge User Guide 5008539 A 5 Laser Light Laser Specifications The SpectraMax® Paradigm® Multi-Mode Detection Platform is rated a Class 1 Laser Product because it houses one or more Laser Modules whereas the laser light is not accessible. Open the catalog to page 1. ISM Equipment Classification (Group 1, Class A) This equipment is. SpectraDrop Abs Protein Quant (i3-i3x)Certified SpectraMax ® M5 e-HTRF Readers The SpectraMax M5 e reader has the same perfor mance specifications as the M5 but is certified for use with Cisbio Bioassays HTRF (Homogeneous Time-Resolved Fluorescence) technology. Structured Review. , Fisher Scienti fi c, cat. i3x Multi-Mode Microplate Reader. The SpectraMax® i3x multi-mode microplate reader is a configurable, versatile microplate. 2 only) SpectraMax M-series Microplate readers. Controls containing all reaction components with either DI water or a fluorescent standard in place of the fluorescent substrate were run alongside the experimental samples. 0112-0115J 9 Chapter1:Introduction TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-ModeSpectraMax i3x and iD5 readers can be equipped with an HTRF detection cartridge or Enhanced TRF module and filters, respectively. Superior flexibility The SpectraMax i3 Multi-Mode Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Filterless readers provide consistent results with steady temperature regulation. SpectraMax i3x and Paradigm readers require the FP-FLUO. Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. SpectraMax Paradigm Platform in conjunction with AlphaLISA provides a powerful tool to screen novel therapeutics in a pathologically relevant, endothelial cell-based model. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. The microplate was then placed in a SpectraMax i3x reader and incubated at 37°C for the duration of the kinetic measurement. 性能特点MD SpectraMax i3X 多功能荧光检测仪为采用光谱融合照明设计、测试功能模块化卡盒设计，具有细胞成像分析单元。. The user guide also covers the features and specifications of the SpectraMax MiniMax 300. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. The SpectraMax M2 and M2e systems provide the multi detection microplate readers with dualmonochromators, dual-mode cuvette ports, with top & bottom reading. Hold down the green power button on the front of the instrument for 5 seconds or until the instrument power down. . I am a person who can handle a range of laboratory and data management tasks with precision and accuracy. Figure 1. This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Microplate reader assays requiring infrared (IR) or red-shifted. Simplified workflow: Add cellular imaging assays in a small footprint. The experiments were performed in triplicates and the spectral reading was recorded using Spectramax i3x microplate reader. 価格、見積もり、お近くの販売店を. SpectraMax Microplate Readers. . Located at the right front of the SpectraMax Plus 384, the cuvette chamber has a lid that lifts up, allowing you to insert or remove a cuvette. SpectraMax i3x 多功能微孔板读板机 SpectraMax Paradigm 多功能微孔板读板机 SpectraMax M5e 多功能微孔板读板机 扫一扫关注我 们的官方微信 Molecular Devices 大中华区 全国咨询服务热线: 400-820-3586 Email: info. . china@moldev. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Reporter Assay. Introduction Inflammation, the body’s reaction to infection, irritation, or other injury, is accompanied by increased endothelial chemokine. SoftMax Pro. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by. Explore a wealth of applications with the future-ready SpectraMax i3x. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. The M5 can read volumes down to 2uL with the uMax Low. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for. 312 gain calibration failed. This technique allows for quality control and assurance when performing surface functionalization of nanoparticles. Ease of use. Danaher Inc spectramax i3x. Spectramax i3 manualSpectramax i3 manual Microplate spectramax i3x molecular biocompareSpectramax i3. TP medium was used as a blank and its fluorescence intensity was subtracted from that of mCherry or from chlorophyll auto-fluorescence intensity. SpectraMax i3x Multi-Mode Detection Platform. HTRF-compatible SpectraMax Multi-Mode Readers. Keywords: fluorescence polarization, spectramax detection cartridge, spectramax paradigm readers, spectramax paradigm, upgredeable cartridge, Created Date: 8/1/2013 10:01:48. For more information on the SpectraMax i3x Multi-Mode Microplate Reader System, please click here. Multimode Readers Comparison Chart. The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. 0´ 104 4. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. The SoftMax® Touch Software is installed in. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. ; India imports most of its Spectramax from United States, China and Austria and is the largest importer of Spectramax in the World. Find regional support. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. 9 x 10-15. The SpectraMax i3x Injector Cartridge with SmartInject Technology is a data sheet that describes the features and benefits of this innovative accessory for the SpectraMax i3x Multi-Mode Detection Platform. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. ai; ImageXpress Pico; IN Carta Image Analysis Software; CLONE SCREENING. com. . In this product video, see how the SpectraMax M5 quickly reads samples. Results for SpectraMax i3x reader with NanoBRET Detection Module (blue) and on-board luminescence detection mode (orange). . were measured at OD405 using a SpectraMax i3x multimode detection system (Molecular Devices, San Jose, CA, USA), and the melanin concentration was determined using the standard curve of melanin solutions with known concentrations. Spectranet customer portal(SpectraMax i3x and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most published microplate reader control and microplate data analysis software package available to scientists. Absorbance Fluorescence (top/bottom read) Luminescence (top read) Time-Resolved Fluorescence (top read). SpectraMax Microplate Reader - Choose from our extensive SpectraMax® line of user-friendly microplate readers. . 1321N1, a human astrocytoma cell line. Molecular Devices社の製品: 吸収度マルチモードマイクロプレートリーダー SpectraMax® i3x に関するすべての情報をご覧ください。. The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. The Molecular Devices SpectraMax i3X Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. SpectraMax M3 Plate Read, Plate Map, Data Analysis and Export Setup pg. Get more information about Fluorescence Polarization SpectraMax Detection Cartridges, which offer great sensitivity for SpectraMax i3x and Paradigm Systems. This eBook shows how to see more and do more with an imaging cytometer that enables rapid imaging and cell analysis. The plate was covered and incubated for one hour at room temperature. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The SpectraMax i3x reader allows one to unravel the mysteries of science by exploring cellular pathways and protein. SoftMax® Pro Software for Windows 10 and 11 is designed to provide the simplicity, flexibility and power required for advanced data analysis. detection cartridge LUM96: 59. Wokingham, Berkshire RG41 5TS United Kingdom. SpectraMax Quant dsDNA Assay Kits. SpectraMax iD3 reader with touchscreen and inserted USB flash drive. Italic and boldface type are used for emphasis. GM2000 Register or Sign In. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. A SpectraMax ® i3x was used to determine uo-rescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. The following detection cartridge is required: • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. . . The NET-specificity of the detected signal was determined by incubation with DNase I (50 U/mL, Qiagen, Germany). The SpectraMax i3x reader provides theSoftMaxProSoftwareFormulaReferenceGuide 8 5049093A SummaryFormulas Summariesconsistoffourparts: ASummaryname(whichisusedtorefertotheSummaryinotherformulas). Insert the snorkel into the bottle and then insert the snorkel into the snorkel clamp. pathways and protein activation and expression in one system. Spectranet customer portalThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. 0´ 104 8. A SpectraMax® i3x was used to determine fluorescence with an excitation wavelength of λ = 360 nm and emission at λ = 460 nm. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Spectranet customer portalSpectraMax M2 & SpectraMax M2e Multi-mode Plat e Readers Operator s Manual 0112-0115 Rev. 13 There are a fairly limited number ofSARS-CoV-2 variants of concern (VOCs) that increase transmission or disease severity or reduce diagnostic or vaccine efficacy continue to emerge across the world. i3x Specifications. Capture Flash Assays with Ease. 58% for the detection module and 2. 304 signal level saturation. 2 x 10-14. A 1:2 dilution series of BSA was prepared in PBS, starting at 100 mg/mL, in LoBind microcentrifuge tubes. 2 nM was calculated using SoftMax Pro Software. Finally, their fluorescence was measured with 365-nm excitation and 450-nm emission filters using a microplate fluorometer (SpectraMax i3x, Molecular Devices, San Jose, CA, USA) (Saiya-Cork et al. SpectraMax iD5:SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. Background Parallax,Columns 2. Features technology from the SpectraMax i3x and MiniMax 300 Imaging Cytometer. SpectraMax i3x readers. The SpectraMax® i3x Multi-Mode Microplate Reader with the SpectraMax® MiniMax™ 300 Imaging Cytometer is equipped with transmitted light (TL), green fluorescence (Ex/Em: 460/541), and red (625/713) fluorescence channels. The ScanLater Western Blot Detection Cartridge is a user-installable option for the SpectraMax i3, i3x and Paradigm Multi-Mode Readers. Spectrophotometer 또는 Microplate Reader 상의 260nm에서 측정된 DNA 샘플의 흡광도는…. ü; ü. 030 fmol. The calculated LOQ values were 0. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. to View Figures. For each assay kit, a preconfigured protocol is included in SoftMax Pro Software to facilitate the acquisition and analysis of data for a simplified workflow. The SpectraMax DuoLuc Reporter Assay has been performance optimized on the SpectraMax i3x reader with Injector Cartridge. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. 6% for the on-board optics (monochromators). Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. 315 can't find zero order. SpectraMax i3/i3x Multi-Mode Microplate Reader SoftMax Pro Software 0 500 1000 1500 2000 2500 3000 0 1´ 106 2´ 106 3´ 106 dsDNA (pg/we l) R F U 02 4 6 8 1 0 2. Monochromator optics with adjustable bandwidth offer excellent sensitivity down to 6 nM tryptophan, as well as precise determination of the fluorescence emission peak shift that occurs upon protein denaturation. Clamp for injector 1 is on the left and clamp for injector 2 is on the right. . 3 STANDARDIZE TO A SINGLE ANALYSIS PLATFORMLearn how you can change the detection module of your SpectraMax i3x instrument in just a few seconds. Real-time measurement of extracellular oxygen depletion and acidification in cultured cells and microorganisms. Wait 20 seconds. The user guide also covers the features and specifications of the SpectraMax MiniMax 300 Imaging Cytometer, an optional accessory that. Leveraging this novel technology, researchers can: Add western blot detection to their microplate reader within 2 minutes. 2 Parallel Line Analysis Conc log [ng/ML] RLU. 10. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening SoftMax® Pro 7. Example: [Drawer]. The SpectraMax® i3x Multi-Mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. 22 μ m fi ltration. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree TechnologyMeasure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. SpectraMax ® iD3 and iD5 Multi-Mode . . The SpectraMax® i3x Multi-Mode Microplate Reader evolves with your future needs and offers an unlimited breadth of application possibilities. Homogeneous and eterogeneous microplate assays can be performed in one flexible system. Description 1. 1 and above:. techsupport. Keywords: htrf spectramax detection cartridge, spectramax i3, spectramax paradigm system, htrf reagents, spectramax i3 and paradigm readers, HTRF assay, htrf. ValitaTiter (i3x i3) ValitaTiter (iD5) ValitaTiter (M5 M5e Flex3) ValitaTiter (Paradigm) ValitaTiter (FilterMax F5) Assay-Specific. SpectraMax i3x Multi-Mode Microplate Reader. Unplug the power cable from the back of the instrument. 303 unable to cal dark current. Multimode Reader Comparison Chart. Background subtraction was not necessary. Current methods available to rapidly detect these can be resource intensive and thus sub-optimal. 50: $75: Improving patient lives by advancing the field of resuscitation medicine. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Molecular Devices推出的SpectraMax® i3x 多功能酶标仪除了具有全波长的光吸收、荧光、化学发光和FRET检测功能以外，还兼容了客户端模块化升级功能，用户可以根据需要任何时间随意升级至荧光偏振FP、HTRF、AlphaScreen、Western Blot、上转发光检测、细胞成像和带有注射器. SpectraMax i3x, onboard luminescence 121. Shop For Laboratory Equipment featuring a wide variety of new, used and refurbished laboratory equipment. 313 reference gain check fail. StakMax Handling Stacker Base Unit. The SpectraMax i3x multi-functional detection platform launched by Molecular Devices can meet the multi-functional detection system with unlimited possible applications as your future needs change. 0200-7029 Wavelength range (LUM): Visible to 650 nm Dead volume: < 10 µL with Reverse . 00800 665 32860. Failed to load PDF file. Cet appareil ISM est confomre à la norme NMB-001 du Canada. #0200-7009) Contact our support team with your questions regarding Molecular Devices products and applications. The AlphaScreen Detection Cartridges utilize a 680nm Laser Diode to provide a sensitive reading system for AlphaScreen assays. Image analysis provides accurate data. Explore a wealth of applications with the future-ready SpectraMax i3x. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A 5 1. SpectraMax® L reader support; ü. User-upgradeable detection modules allow you to expand the capabilities of your microp. Molecular Devicesの全商品を見る. Molecular Devices Software. *On the SpectraMax i3x reader only. Price from $9. KEY FEATURES • Combine cellular imaging and microplate assays into a workflow • Eliminate cell staining for cell counting and confluency with StainFree Technology Measure oxidative metabolism and glycolytic activity on the SpectraMax i3x reader. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers Operator’s Manual — 0112-0115 Rev. Bioz Stars score: 98/100, based on 1 PubMed citations. 2 software (Stat-Ease) to identify factors that have significant effects on cell growth or IgG production, either alone or in combination with. Computer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. Microplate optimization should be performed the first time the assay is run, and subsequently for . HTRF is a proprietary time-resolved fluoresce nce technology that overcomes With these results, as well as CV values below 6% for all standards, both the SpectraMax i3x and the SpectraMax iD5 readers demonstrate high sensitivity and a wide dynamic range for the IP-One assay. Find regional support. INTRODUCTION The SpectraMax M2 and SpectraMax M2e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellEvery SpectraMax ® Paradigm Detection Cartridge starts with an application optimized excitation energy source either in the form of a long life, high powered LED, xenon flash lamp, or high powered laser. SoftMax Pro GxP software is a special release that includ es full user and permission control and audit trail generation in addition to all the dataSpectraMax i3x Multi-Mode Microplate Reader. . Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. The SpectraMax i3x Multi-Mode Microplate Reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. SpectraMax i3x 和 SpectraMax iD5 微孔板读板机上使用 SoftMax Pro 软件中的预设方法进行测量(有关仪器特定设 置，请参见表 2 和表 3 )。在 SpectraMax i3x 和 SpectraMax iD5 读板机上进行了微孔板优化和读取高度调整，以确保最佳 的检测灵敏度和动态范围。 数据分析We would like to show you a description here but the site won’t allow us. Spectramax I3x, supplied by Danaher Inc, used in various techniques. These robust readers have been placed in labs from Antarctica to the. The chamber. 99 to $1999. D 1 1. The SpectraMax MiniMax 300 Imaging Cytometer option is a field-upgradeable option for the SpectraMax i3/i3x Multi-Mode Detection Platform that offers cellular imaging capability. 314 low lamp level warning. The dynamic range of the assay is about 3. The SpectraMax i3x reader grows with you as you continue to advance your research, enabling you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. The plate was then read on the SpectraMax iD5, SpectraMax i3x, and SpectraMax M5e readers using the settings in Table 1. The heating element design of the category 1 microplate readers, such as the SpectraMax i3x Multi-Mode Microplate Reader, applies a dual area sensor for temperature control. TaqMan SNP. This document is copyright protected and any reproduction of this document, in whole or any part, is strictly prohibited, except as Molecular Devices may authorize in. 99. ImageXpress Confocal HT. Description. 4. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Biochrom New EX Read 400 Microplate Reader. . Figure 1. 012 fmol 0. HeLa cells were plated at 15,000 cells per well in 100 µL. user upgradeable. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. EUROPE +44-118-944-8000 Mon to Fri 8am - 5pm GMT. VAL014, right table) assays on the SpectraMax i3x reader are shown below. ImageXpress Confocal HT. Core features include biological and fume hoods, a cold room and multiple freezers, and access to institutional space for radiolabeling and radiation monitoring. Molecular Devices SpectraMax i3x Multi-Mode Microplate Platform. Because a PMT needs to be set to a specified gain to reach a specific sensitivity,MDC instruments including: SpectraMax®, VersaMax®, VMax®, EMax®, Gemini, ThermoMax®, and FlexStation®. Note: Additional readers with similar performance are listed. Molecular Devices are known for both the reliability and the wide uses of their products. Auto PMT Challenge: A standard Photomultiplier Tube (PMT) or detector limits the dynamic range of your instrument. 1. Using the SoftMax Pro Workflow Editor, a kinetic cycle containing an absorbance read (PlateOD600) and a fluorescence read (PlateGFPBottom) with a 5-second linear plate shake between reads was created (Figure 1). 99. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploringFluorescence at 365 nm excitation and 450 nm emission filters was determined based on a microplate fluorometer (SpectraMax i3x, Molecular Devices, Beckman Coulter, CA, USA). Published Dec 16, 2020 | Updated Apr 13, 2023. Examples: “Press carefully to engage,” “ Do not. HTRF IP1 standard curve measured on the SpectraMax i3x (green circles) and SpectraMax iD5 (red circles) readers. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. . Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader and Thermo Fisher Scientific’s Varioskan LUX reader also perform multiplexing. Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the field-upgradeable SpectraMax® MiniMax™ 300 Imaging Cytometer enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression. Microplate readers, multi-mode, SpectraMax® i3x. 800. (3) Molecular Devices, SpectraMax i3x, Microplate Readers (2) 2022 (1) 2020 Invetech Korus KRS-100 Elutriation & Washing Systems (3) 2021 ThermoFisher Scientific, invitrogen, CytKick Max AutoSampler w/ Attune NxT: Acea NovoCyte Flow Cytometer: Appliedbiosystems, QuantStudio 7 Flex, Real-Time PCR SystemThe SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Prepare your i3/i3x instrument for shipment. ü; SpectraMax® Mini reader support. The 4x washing steps were also performed on deck using the Span-8 aspirate and dispense for the first three washes. to View Figures. Find regional support. The SpectraMax Injector Cartridge enables precise reagent delivery and adjustment of delay and integration times that are required to obtain optimal sensitivity for this assay. Transcreener® HTS is a universal, high throughput biochemical assay platform based on the detection of nucleotides, which are formed by thousands of cellular enzymes—many of which catalyze the covalent regulatory. 58% for the detection module and 2. SpectraMax Microplate Readers. Sensitive TRF detection of two simple, mix-and-measure assay workflows. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. Find regional support. 5mM EDTA, 1% NP40,Explore a wealth of applications with the future-ready SpectraMax i3x. g. The SpectraMax i3x Multi-Mode Detection Platform User Guide provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax i3x, a versatile and customizable microplate reader that can perform various assays and imaging tasks. Shaking and incubations were performed using an on-deck orbital shaker. SpectraMax i3x Register or Sign In. The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence,. . This update is applicable for users of the SpectraMax® i3x Multi-Mode Detection Platform and SpectraMax® Paradigm® Multi-Mode Microplate Reader with the ScanLater Western Blot (WB) Detection Cartridge and the SpectraMax® iD5 Multi-Mode Microplate Reader: SMP 7. Monday to Friday 7am - 5pm Pacific Time. The SpectraMax i3x reader measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast kinetics with injectors. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring Download SoftMax® Pro 7. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. Results were plotted as ratio of green/red vs. User Manuals, Guides and Specifications for your Molecular Devices SpectraMax i3x Measuring Instruments. 306 plate air cal fail,low light. The SpectraMax® i3 Multi-Mode Microplate Reader Detection Platform evolves with your future research needs and offers a breadth of application possibilities. The possibility of user upgrading the system with an application cartridge and an imaging cytometer makes this device the most flexible microplate. Examples: “Press carefully to engage,” “ Do not press down. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. Keywords: filtermax multi mode microplate readers, spectramax paradigm multimode detection platform, spectramax paradigm system Created Date: 11/16/2010 12:21:51 PMThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. SpectraMax MiniMax 300 细胞成像系统 为 SpectraMax i3x 多功能微孔板检测平台而打造！ • • • • 主要特点 系统全面兼容细胞成像 及微孔板检测技术 独特无标记分析技术无需 对细胞染色即可进行细胞 计数和贴壁度的计算 具有多种检测功能可对 细胞活力及毒性进行全. applications, including dual luciferase and ATP assays. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast. Dual auto injector cartridges for the SpectraMax® i3x Multi-Mode Microplate Detection Platform allows you to expand your research capabilities to include flash based applications, including dual luciferase and ATP assays. Prime function 20 amol. If no sound is heard during initialization, contact technical support. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. FTIR Spectrometer: Shimadzu IRSpirit (2018)The plate was read on the SpectraMax i3x Multi-Mode Microplate Reader. . Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part. 001 0. SpectraMax i3x; SpectraMax iD3 / iD5; SpectraMax M Series; SoftMax Pro Software; CELLULAR IMAGING. HTRF IP standard curve. The SpectraMax i3x is a multi-mode microplate reader that measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western. The SpectraMax i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one system. Also review the Maintenance and Troubleshooting chapter of the user guide for your model plate reader. SpectraMax i3x microplate reader and SoftMax Pro Software protocol. 22mm 384 FilterMax™F3&F5Multi-ModeMicroplateReaders,SpectraMax®i3x Multi-ModeDetectionPlatform,SpectraMax®iD3&iD5Multi-Mode MicroplateReaders,andSpectraMax®Paradigm®Multi-Mode MicroplateReader 5301193 18mm 96 SpectraMaxi3x,SpectraMaxiD3,SpectraMaxiD5,SpectraMaxM. Unparalleled performance on a personalized platform. 99. Slide the tip into the nozzle, the slots are labeled 1 and 2 for injector 1 and injector 2. 58% for the detection module and 2. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your initial investment. 0001 0. Read more about HTRF SpectraMax Detection Cartridges for SpectraMax i3x & Paradigm Systems which utilize a high energy Xenon flash lamp for the sensitive reading of HTRF reagents. 3 Figures from 612 Publications Promega. User-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Molecular Devices, LLC Reading Modes: Endpoint and Kinetic; Optical System: Spectral Fusion Illumination. D 1 1. Download PDF. 4. 5 µL anti-cAMP-Cryptate. pH-Xtra Glycolysis Assay - Agilent. Price from $9. BRET2canbeusedtostudyvariouskindsofprotein-proteininteractions. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Faster data acquisition and analysis with preconfigured SoftMax Pro protocols. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. Capabilities include fluorescence and luminescence readings, environmental control, cell viability assays, enzyme. SpectraMax i3x plate reader (Molecular Device, contact supplier directly) Vacuum pump system (e. Sufficient for 1000 reactions. Curcumin binding assay. Adding time resolved fluorescence, HTRF® or western blot detection simply requires the user to exchange a detection module. Each heating element is independently. Instrument. Both readers feature an 8-channel read head for fast reads and. • SpectraMax i3x Injector Cartridge with SmartInject™ Technology • Expand your research capabilities to include flash-based . The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 0´ 105 dsD NA (p g/ wel ) R F UFluorescence intensity was measured using a Spectramax i3x plate reader (Molecular Devices) with excitation at 575 nm (bandwidth 9 nm) and emission at 608 nm (bandwidth 15 nm). In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. 9000-0756. SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 72 5039228D. For more detailed information and technical support of Molecular Devices instruments or software,The SpectraMax i3x Multi-Mode microplatereader measures spectral-based Absorbance, Fluorescence, and Luminescence with the added functionality of modular upgrades for Western Blot, Imaging and Fast Kinetics with Injectors. Bioz Stars score: 98/100, based on 1 PubMed citations. Both readers demonstrated their ability to detect the HTRF signal from the terbium donor and red acceptor combination, and they could be used for saturation studies using the Tag-lite technology. One sensor measures the temperature of the top chamber heating foil, and another is used to measure the bottom chamber heating foil. All the readers were operated using SoftMax® Pro Software, which also has built-in data analysis and curve-fitting for automatic calculation of EC 50. application modules • Multi-mode microplate reader and imaging system • Wide dynamic range • StainFree™ cell counting technology • Library of user-configurable applications • Advanced curve fitting and statistical analysis • Cell confluence and cell viabilitySoftMax® Pro 7. SeeThe SpectraMax i3x measures monochromator-based absorbance, fluorescence and luminescence with a future-ready platform that can evolve with the researcher’s needs. Bioz Stars score: 98/100, based on 1 PubMed citations. . As. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. Table 1.